ADHESION PROTEIN PROTOCOLS
Elisabetta (Mario Negri Institute, Milan, Italy)
Corada, Monica (Mario Negri Institute, Milan, Italy)
international corps of expert investigators describe their optimized techniques
for both the identification of new cell adhesion proteins and for the
characterization of novel adhesive structures. Their cutting edge methods will
enable both novice and experienced researchers readily to identify and clone new
adhesion proteins, to successfully produce inhibitors of the adhesive activity,
to develop biological models for the assay of cell-to-matrix and cell-to-cell
adhesion in vitro, and to measure the signaling activities of adhesion proteins.
By illuminating these adhesive molecules and the possibilities for manipulating
them, the new experimental strategies collected here will have considerable
clinical potential for the regulation of immunity, inflammation, tissue
remodeling, and embryonic development.